植物原生质体全能性表达及其在甘蓝类蔬菜育种上的应用盛小光等(浙江省农业科学院蔬菜研究所,浙江杭州310021)—《中国蔬菜》2011(16)植物原生质体指植物除去细胞壁后被质膜包被的裸露细胞,在适当培养条件下具有再生成完整植株的全能性。植物原生质体分离、培养及植株再生技术是进行植物育种、基因工程、遗传理论及细胞生理特性研究的平台,具有重要的研究意义。本文概述了植物原生质体全能性表达过程中原生质体的获得、培养和再生等关键技术环节及其在甘蓝类蔬菜育种 Plant Protoplast Totipotentiality Expression and Its Application in Brassica Vegetable Breeding SHENG Xiaoguang et al (Vegetable Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China) - “Chinese Vegetables” 2011 (16) Plant protoplast refers to the plant The membrane-coated naked cells have the pluripotency that regenerates the intact plant under suitable culture conditions. Plant protoplast isolation, culture and plant regeneration technology is a platform for plant breeding, genetic engineering, genetic theory and cell physiological characteristics, has important research significance. This paper summarizes the key technologies of protoplast acquisition, culture and regeneration in the process of plant protoplast expression and its application in cabbage breeding