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目的通过细胞模型和人群研究,探讨血清δ-氨基乙酰丙酸脱水酶(ALAD)含量在铅暴露人群中的分布情况,为铅中毒早预防、早诊断提供依据。方法 Real-time RT-PCR检测铅处理HEK293细胞模型中ALAD变化情况;ELISA方法检测铅暴露人群和非铅暴露人群中血清ALAD、游离红细胞原卟啉(FEP)和平均红细胞血红蛋白量(MCH),分析其在铅中毒过程中的变化情况。结果 HEK293细胞铅处理后ALAD基因mRNA表达水平显著下降(P<0.001);人群中ALAD和MCH含量与血铅浓度呈负相关关系(P<0.001),ALAD和FEP呈负相关关系(P<0.001),且ALAD含量在血铅浓度>300μg/L及≤300μg/L时差异有统计学意义。结论 ALAD作为铅中毒的一个敏感指标,可为职业性铅中毒早期预防和诊断提供某些依据,为铅中毒新标准制定提供一定参考。
Objective To investigate the distribution of δ-aminolevulinic acid dehydratase (ALAD) in lead exposure population by cell model and population study to provide basis for early prevention and early diagnosis of lead poisoning. Methods Real-time RT-PCR was used to detect the changes of ALAD in lead-treated HEK293 cells. The serum ALAD, free erythrocyte protoporphyrin (FEP) and mean erythrocyte hemoglobin (MCH) were measured by ELISA. Analysis of the changes in the lead poisoning process. Results The levels of ALAD mRNA in HEK293 cells were significantly decreased (P <0.001). The levels of ALAD and MCH in blood were negatively correlated with blood lead levels (P <0.001) and negatively correlated with ALAD and FEP ), And ALAD content in the blood lead concentrations> 300μg / L and ≤ 300μg / L, the difference was statistically significant. Conclusion ALAD as a sensitive indicator of lead poisoning can provide some evidence for the early prevention and diagnosis of occupational lead poisoning and provide a reference for the new standard of lead poisoning.