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使用ＰＣＲ技术对１４份广西ＨＩＶ－１阳性感染者外周血单核细胞（ＰＢＭＣｓ）样品进行扩增，获得ＨＩＶ－１膜蛋白（ｅｎｖ）基因的核酸片段，并对其Ｃ２－Ｖ３及邻区３５０－４５０个核苷酸序列进行了测定和分析。结果表明，１４份样品中９份为泰国Ｂ（Ｂ′）亚型，５份为Ｅ亚型毒株。其中Ｂ′亚型毒株的基因离散率为４．２％，与Ａ－Ｅ参考亚型及部分Ｂ亚型代表株序列相比较，与包括泰国、缅甸及云南德宏在内的Ｂ亚型毒株序列十分接近，相互之间基因离散率在３．０％－４．４％的范围内；而Ｅ亚型毒株的基因离散率为２．１％，与国际Ｅ亚型毒株的基因离散率最近，为５．６％，与其它国际参考亚型基因离散率很远，在２１．１％－２７．３％。根据以上数据及其它资料提示，广西存在Ｂ′和Ｅ两种亚型的ＨＩＶ－１的流行，且其Ｂ′亚型毒株的传入，与流行在云南德宏州的相同亚型ＨＩＶ－１毒株密切相关，而Ｅ亚型毒株则可能是由泰国经越南传入广西的
A total of 14 HIV-1 positive peripheral blood mononuclear cells (PBMCs) from Guangxi were amplified by polymerase chain reaction (PCR), and the HIV-1 membrane protein (env) -450 nucleotide sequences were determined and analyzed. The results showed that 9 of the 14 samples were B (B ’) in Thailand and 5 were subtypes of E subtype. Among them, the genetic divergence rate of B ’subtype was 4.2%. Compared with the A-E reference subtype and the partial subtype B representative strain, the B subtype including Thailand, Burma and Yunnan Dehong The sequences of the strains were very close and the genetic dispersions among them were in the range of 3.0% -4.4%. The genetic divergence rate of the strains of subtype E was 2.1% The gene dispersion rate is 5.6% recently, which is far away from other international reference subtype gene dispersions at 21.1% -27.3%. According to the above data and other data suggest that there is Guangxi B ’and E two subtypes of HIV-1 epidemic, and its B’ subtype strains of the incoming, and popular in Yunnan Dehong Prefecture of the same subtype of HIV- 1 strains are closely related, while the E subtype strains may be from Thailand through Guangxi into Guangxi