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目的：建立胶囊微量法１４Ｃ－尿素呼气试验（１４Ｃ－ＵＢＴ）用于诊断幽门螺杆菌（Ｈｐ）感染。方法：采用ｌμＣｉ１４Ｃ－尿素，胶囊给药，在收集５，１０，２０，２５，３０，４５，６０ｍｉｎ呼气样本的基础上建立了以１４ＣＯ２呼出高峰期为２５ｍｉｎ，直接用１６．６７ｍＢｑ／ｍｍｏｌ表示的检测方法，结果与Ｈｐ细菌学培养及病理组织学比较。结果：共检测１３７例病人，Ｈｐ阳性８３例，阴性５４例。以Ｈｐ阴性第２５分钟１４ＣＯ２放射量均值±３ｓ作为判断Ｈｐ感染的临界值（２８０ｄｐｍ），结果显示敏感性９７．０６％，特异性９５．１２％，阳性预检值９７．０６％，阴性预检值９５．１２％。结论：胶囊微量法１４Ｃ－ＵＢＴ具有无创、安全、快速、准确、简便、经济等优点，可以代替传统的５μＣｉ～１０μＣｉ；大剂量１４Ｃ－ＵＢＴ。
Objective: To establish a 14C-urea breath test (14C-UBT) for the diagnosis of Helicobacter pylori (Hp) infection. Methods: Using lμCi14C-urea, capsule administration, on the basis of collecting breath samples of 5, 10, 20, 25, 30, 45, 60min, the peak of 14CO2 exhaled was set as 25min and 16.67mBq / mmol The detection results were compared with those of Hp bacteriological culture and histopathology. Results: A total of 137 patients were detected, Hp-positive in 83 cases, negative in 54 cases. The mean value of 14CO2 radioactivity ± 3s during the 25th minute of Hp negative was taken as the critical value for detecting Hp infection (280dpm). The results showed that the sensitivity was 97.06%, the specificity was 95.12% and the positive value was 97.06% Check 95.12%. Conclusion: The 14C-UBT capsule has the advantages of noninvasive, safe, rapid, accurate, simple, economical and can replace the traditional 5μCi ~ 10μCi; high-dose 14C-UBT.