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应用淋巴细胞丝裂原刺激后细胞~3H-TdR参入的方法,研究了小剂量丁射线照射(0～4cGy)诱导淋巴细胞转化功能的适应性反应;探讨了影响适应性反应表现的几个因素,即D_1剂量、D_2剂量及D_1和D_2照射的时间间隔。结果表明,体外培养24h的人外周血淋巴细胞在小剂量丁射线(0.5～4.0cGy)照射后,均产生了适应性反应,并以1.0cGy的γ射线照射所诱导的适应性反应最强。随剂量的增大,适应性反应减弱,说明了小剂量辐射诱导适应性反应存在着最适剂量范围。D_1(1.0cGy)照射后6,24,48,72h照射D_2(3Gy),结果表明24h的间隔适应性反应最强,这与照后细胞的增殖周期有关。1～7Gy的D_2照射,均可观察到适应性反应,而以3.0Gy照射后反应最强。D_2剂量过大,适应性反应不明显,剂量过小,适应性反应显现不充分,说明D_2剂量在适应性反应中亦有最适剂量范围。
The application of 3H-TdR incorporation in cells stimulated with lymphocyte mitogen was used to study the adaptive response of lymphocyte transformation induced by small dose of Ding-ray irradiation (0-4cGy). Several factors affecting the performance of adaptive response , Namely D_1 dose, D_2 dose and D_1 and D_2 irradiation time interval. The results showed that all the human peripheral blood lymphocytes cultured in vitro showed adaptive response after being irradiated with small doses of Ding rays (0.5-4.0 cGy), and the strongest adaptive responses were induced by 1.0 cGy gamma rays. With the increase of dose, the adaptive response weakened, indicating that there is an optimum dose range for low dose radiation induced adaptive response. D 2 (3Gy) irradiation at 6,24,48,72h after D 1 (1.0cGy) irradiation showed that 24h interval adaptive response was the strongest, which was related to cell proliferation cycle after irradiation. 1 ~ 7Gy D_2 irradiation, adaptive response can be observed, and the strongest response after 3.0Gy irradiation. D 2 dose is too large, the adaptive response is not obvious, the dose is too small, the adaptive reaction appears inadequate, indicating D_2 dose in the adaptive response also has the optimum dose range.