论文部分内容阅读
兴奋-收缩偶联是指肌细胞的电激动经过转导引起肌细胞收缩等一系列级联反应和信息传递过程.心肌细胞作为肌细胞的一种,它的兴奋-收缩偶联是心脏正常生理功能的基础.电兴奋和机械收缩作为ECC两个标志性的生理过程,其作用呈双向性,任何一阶段的异常都会导致心肌细胞ECC障碍,深入探讨和研究两者的相互作用为ECC异常导致的心律失常等心脏疾病的预防和治疗提供了新思路,寻求更为简便和有效的检测心肌细胞电兴奋和机械收缩的方法已成为研究者的一个热门研究课题.细胞芯片作为一种无损的实时传感检测手段,在生命科学、环境监测和药物开发等领域有着广泛的应用.本文首先概述了心肌细胞的ECC机制,接着针对国际上心肌细胞电兴奋和收缩检测领域最新研究成果,分析了多种芯片检测方法应用于心肌细胞ECC检测的可行性以及面临的问题,并介绍了一种实验室自主研发的多尺度电极阵列芯片.该芯片在不同电极位点分别记录细胞的电兴奋和机械收缩,可对ECC进行系统的分析,最后结合该芯片对芯片技术的应用前景进行了展望.
Excitation - contraction coupling refers to a series of cascade reactions and information transfer processes that result from the electrical activation of muscle cells through the transduction of muscle cells.As a kind of myocyte, cardiomyocytes, its excitement - contraction coupling is normal cardiac physiology Function of the foundation.Electric excitement and mechanical contraction as ECC two iconic physiological processes, the role of a two-way, any one of the abnormalities will lead to ECC cardiomyopathy, in-depth exploration and study of the interaction between the two as an abnormal result of ECC And provide a new idea for the prevention and treatment of cardiac diseases such as cardiac arrhythmia.It has become a hot research topic for researchers to find a more convenient and effective method to detect electrical excitability and mechanical contraction of cardiomyocytes.As a nondestructive real-time Sensing means has a wide range of applications in the fields of life sciences, environmental monitoring and drug discovery.In this paper, the mechanism of ECC in cardiomyocytes is firstly outlined, and then the latest research results in the field of electrical excitations and contractions of cardiomyocytes in the world are analyzed. Chip chip detection methods applied to the detection of myocardial cells feasibility and problems faced by ECC, and introduced a Laboratory independently developed multi-scale electrode array chip. The chips are recorded at different electrode sites excitability and mechanical contraction cells, may be of the ECC system analysis, the final binding of the chip prospect chip technology prospected.