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为了探讨马铃薯抗黑痣病机制,采用毒素接种脱毒组培苗的方法,研究了黑痣病菌毒素对马铃薯不同抗性品种幼苗体内病程相关蛋白、木质素及游离脯氨酸含量的影响及其与抗黑痣病的关系。毒素处理后,感病品种大西洋和抗病品种底西芮幼苗几丁质酶和β-1,3-葡聚糖酶活性均明显升高,36 h升高幅度均达到最大,感病品种升幅大于抗病品种。底西芮和大西洋木质素及游离脯氨酸含量也大幅增加,但抗病品种的增加幅度大于感病品种。研究表明,黑痣病菌毒素诱导几丁质酶和β-1,3-葡聚糖酶活性升高以及木质素和游离脯氨酸含量增加是马铃薯幼苗抵抗毒素胁迫的内在机制;木质素、游离脯氨酸含量与品种抗病性呈正相关,而几丁质酶、β-1,3-葡聚糖酶活性变化则不是引起品种抗性差异的主要原因。
In order to investigate the mechanism of resistance to black nematode in potato, the effects of Neisseria gonorrhoeae toxin on the content of disease-related proteins, lignin and free proline in seedlings of different resistant varieties of potato And anti-mole disease relationship. After toxin treatment, the activities of chitinase and β-1, 3-glucanase in the susceptible cultivar Atlantic and resistant variety Boshiri seedlings were significantly increased, and reached the maximum at 36 h. The susceptible varieties increased Greater than the disease-resistant varieties. The content of lignin and free proline also increased significantly in bottom west and Atlantic, but the increasing range of resistant varieties was more than that of susceptible varieties. Studies have shown that the pathogenesis of potato seedlings resistant to toxin stress is induced by C. viridis toxin induced chitinase and beta-1, 3-glucanase activity and lignin and free proline content increased; lignin, free The proline content was positively correlated with the disease resistance of the cultivars, while the changes of chitinase and β-1, 3-glucanase activities were not the main reasons for the differences in resistance.