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AIM:To assess the expression of cydooxygenase-2(COX-2),nitric oxide synthase(iNOS),p53 and Ki-67 in gastric mucosa-associated lymphoid tissue(MALT)lymnphoma and clarifythe relationship between COX-2 expression and iNOS orp53 expression in these patients.METHODS:The expressions of COX-2,iNOS,p53 and Ki-67were detected in 32 gastric MALT lymphoma specimensand 10 adjacent mucosal specimens by immunohistochemicalEnvision method.RESULTS:COX-2 and iNOS expressions were significantlyhigher in gastric MALT lymphoma tissues than those inadjacent normal tissues.The expression of COX-2 wasobserved in 22 of 32 cases of MALT lymphoma tissues(68.8%).A positive cytoplasmic immunoreactivity for iNOSwas detected in 17 of 31 cases(53.1%).COX-2 expressionin gastric MALT lymphoma tissues was positively correlatedwith iNOS expression(r=0.448,P=0.010)and cell proliferativeactivity analyzed by Ki-67 labeling index(r=0.410,P=0.020).The expression of COX-2 protein did not correlate with age,sex,stage of disease,lymph node metastasis or differentiation.The accumulation of p53 nuclear phosphoprotein was detectedin 19(59.4%)of tumors,p53 protein was expressed in 11of 23 assessed LG tumors and in 8 of 9 assessed HG tumors.The difference of p53 positivity was found statisticallysignificant between LG and HG cases(P=0.0302).The p53accumulation correlated with advanced clinical stage(stageⅢ+Ⅳ vs stage Ⅰ+Ⅱ,P=0.017).There was a significantpositive correlation between COX-2 expression and p53accumulation status(r=0.403,P=0.022).The mean PI ofKi-67 in each grade group were 36.0±7.73% in HG and27.4±9.21% in LG.High-proliferation rate correlated withHG tumors(r=0.419,P=0.017).The correlation coefficientshowed a significant positive correlation between PI andCOX-2 expression in MALT lymphoma patients(r=0.410,P=0.020).CONCLUSION:COX-2 expresses in the majority of gastricMALT lymphoma tissues and correlates with cellularproliferation and iNOS expression.COX-2 overexpressionis closely associated with p53 accumulation status,iNOSand COX-2 may play a synergistic role in the pathogenesisof gastric MALT lymphoma.
AIM: To assess the expression of cydooxygenase-2 (COX-2), nitric oxide synthase (iNOS), p53 and Ki-67 in gastric mucosa-associated lymphoid tissue (MALT) lymnphoma and clarify the relationship between COX-2 expression and iNOS orp53 expression in these patients. METHODS: The expressions of COX-2, iNOS, p53 and Ki-67were detected in 32 gastric MALT lymphoma specimensand 10 adjacent mucosal specimens by immunohistochemical Envision method .RESULTS: COX-2 and iNOS expressions were significantlyhigher in gastric MALT lymphoma tissues than those in adjacent normal tissues. The expression of COX-2 wasobserved in 22 of 32 cases of MALT lymphoma tissues (68.8%). A positive cytoplasmic immunoreactivity for iNOS was detected in 17 of 31 cases (53.1%). MALT lymphoma tissues were positively correlated with iNOS expression (r = 0.448, P = 0.010) and cell proliferative activity analyzed by Ki-67 labeling index (r = 0.410, P = 0.020). The expression of COX-2 protein did not correlate with age, sex, stage of diseas The lymph node metastasis or differentiation. The accumulation of p53 nuclear phosphoprotein was detected in 19 (59.4%) of tumors, p53 protein was expressed in 11 of 23 of the 23 of HG tumors. The difference of p53 positivity was found The p53accumulation correlated with advanced clinical stage (stage Ⅲ + Ⅳ vs stage Ⅰ + Ⅱ, P = 0.017). There was a significant positive correlation between COX-2 expression and p53accumulation status (r = 0.403, P = 0.022). The mean PI of Ki-67 in each grade group was 36.0 ± 7.73% in HG and 27.4 ± 9.21% in LG. High-proliferation rate correlated with GH tumors (r = 0.419, P = 0.017). The correlation coefficient showed a significant positive correlation between PI and COX-2 expression in MALT lymphoma patients (r = 0.410, P = 0.020) .CONCLUSION: COX-2 expresses in the majority of gastricALTALT lymphoma tissues and correlates with cellularproliferation and iNOS expression. COX- 2 overexpressionis closely associated w ith p53 aaccumulation status, iNOS and COX-2 may play a synergistic role in the pathogenesis of gastric MALT lymphoma.