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目的:探讨阿奇霉素对地高辛在小肠的吸收及其对小肠P-糖蛋白表达的影响。方法:采用外翻小肠囊法收集透膜液样本,肠道给药后采集血浆样本,用荧光偏振免疫测定(FPIA)法测试透膜液和血浆中地高辛浓度;用Western blot法测定P-糖蛋白的表达。结果:合用阿奇霉素后,地高辛小肠透膜量大幅度增加,血浆中地高辛浓度升高,P-糖蛋白的表达明显降低。结论:阿奇霉素对地高辛的肠道吸收具有明显的增强作用,这种相互作用与小肠P-糖蛋白药泵受到抑制有关。
Objective: To investigate the absorption of digoxin in small intestine and its effect on the expression of P-glycoprotein in small intestine. Methods: Samples of transmembrane fluid were collected by valgus small bowel sac method and plasma samples were collected after intestinal administration. Fluorescence polarization immunoassay (FPIA) was used to measure the concentration of digoxin in plasma and plasma. The levels of P Glycoprotein expression. Results: After combined with azithromycin, digoxin significantly increased the amount of small intestine, plasma digoxin concentration, P-glycoprotein expression was significantly reduced. Conclusion: Azithromycin has a significant enhancing effect on the intestinal absorption of digoxin. This interaction is related to the inhibition of intestinal P-glycoprotein pump.