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目的探讨已发生凝血的血液样本采用超声处理的必要性和最佳时间。方法抽取500名驾驶员血液样本各两份,其中1份为抗凝血,1份为未抗凝血。抗凝血样本采用直接进样,未抗凝血分别采用旋涡震荡后直接进样和进一步以不同时间超声处理后再进样,采用GC法分析各样本中乙醇浓度。以抗凝血检测结果为标准值,进行比较和分析。结果 500份抗凝血液样本中有462份检出乙醇,相应462份未抗凝血液样本中445例检出乙醇。未抗凝血经60min超声处理后再进行GC检测,所得结果与相应抗凝血相符,而采用更长、更短时间的超声处理,或不加超声处理的检测结果绝大多数与相应抗凝血检测值存在显著性差异(P<0.01)。结论机动车驾驶员血液中乙醇浓度测定应使用抗凝血样本;如样本未加抗凝剂出现凝血后,则应对样本进行超声波处理,时间以60min为宜。
Objective To investigate the necessity and optimal time of ultrasonic coagulation of blood samples that have occurred. Methods Two blood samples from 500 pilots were collected. One of them was anticoagulated and the other was non-anticoagulated. Anticoagulant samples were injected directly and no anticoagulated blood was injected directly after vortex and then further sonicated at different times before injection. The concentrations of ethanol in each sample were analyzed by GC. The anticoagulant test results as a standard value for comparison and analysis. RESULTS: Of the 500 anticoagulated blood samples, 462 were detected for ethanol and 445 for 462 non-anticoagulated blood samples were detected for ethanol. After 60min ultrasonic treatment without anticoagulation and then GC detection, the results consistent with the corresponding anticoagulant, and using a longer, shorter time ultrasound treatment, or without ultrasonic treatment most of the test results with the corresponding anticoagulation There was a significant difference in blood test (P <0.01). Conclusion The concentration of ethanol in the blood of motor vehicle drivers should be measured by anticoagulant samples. If coagulation is not observed with anticoagulants, the samples should be sonicated for 60 min.