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目的:原发性腹膜后脂肪肉瘤是一种少见的恶性肿瘤,发病率大约占全部恶性肿瘤的1%,但其却是腹膜后最常见的软组织肿瘤。通过研究原发性腹膜后脂肪肉瘤和正常脂肪组织间的基因表达差异,可以寻找到与原发性腹膜后脂肪肉瘤发生、发展相关的候选基因并为其防治研究提供线索。方法:应用基因芯片技术检测2例原发性腹膜后脂肪肉瘤和相应的正常脂肪组织全基因组序列,分析其基因表达差异。结果:两组间的差异表达基因3828个,其中表达上调有1837个基因,表达下调有1991个基因。其中涉及细胞增殖、黏附、凋亡以及信号通路等多种生物学过程。结论:通过基因表达谱芯片筛选出与原发性腹膜后脂肪肉瘤发生和进展密切相关的基因为其基础研究及临床早期诊断、预防和治疗提供潜在的分子标记和靶基因。
PURPOSE: Primary retroperitoneal liposarcoma is a rare malignancy, accounting for about 1% of all malignancies, but it is the most common retroperitoneal soft tissue tumor. Through studying the difference of gene expression between primary retroperitoneal liposarcoma and normal adipose tissue, we can find candidate genes related to the occurrence and development of primary retroperitoneal liposarcoma and provide clues for its prevention and treatment. Methods: Genomic chip was used to detect the whole genome sequence of two cases of primary retroperitoneal liposarcoma and corresponding normal adipose tissue, and the difference of gene expression was analyzed. Results: There were 3828 differentially expressed genes between the two groups, of which 1837 genes were up-regulated and 199 genes were down-regulated. These involve many biological processes such as cell proliferation, adhesion, apoptosis, and signaling pathways. Conclusion: Genes that are closely related to the occurrence and progression of primary retroperitoneal liposarcoma are screened by gene expression microarray to provide potential molecular markers and target genes for their basic research and early clinical diagnosis, prevention and treatment.