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利用荧光探针和荧光素，研究了乌贼墨对Ｈ_(２２)癌细胞内 Ｃａ~（２＋）、ＡＴＰ浓度及线粒体Ｃａ~（２＋）／Ｍｇ~（２＋）－ＡＴＰ酶活性的影响。结果发现，乌贼墨使Ｈ_(２２)癌细胞内Ｃａ~（２＋）浓度降低了２．３和３．７倍，线粒体Ｃａ~（２＋）／Ｍｇ~（２＋）－ＡＴＰ酶活性降低了 ２８％和 ５８％，ＡＴＰ浓度升高 Ｔ ７７％和 ８３％。结果提示乌浓墨可能通过降低细胞内Ｃａ~（２＋）浓度，继而影响到线粒体上Ｃａ~（２＋）依赖性Ｃａ~（２＋）／Ｍｇ~（２＋）-ＡＴＰ酶活性，减少了Ｃａ~（２＋）向线粒体内的转运，减轻甚至消除了Ｃａ~（２＋）对ＡＴＰ合成的抑制作用。这可能是乌贼墨抑制肿瘤生长的机制之一。rrrrrrrrrn
Using fluorescent probes and fluorescein, the effects of sepia on the Ca~(2+), ATP concentration and mitochondrial Ca~(2+)/Mg~(2+)-ATPase activity in H_(22) cancer cells were studied. The results showed that the squid ink reduced the intracellular Ca 2+ concentration of H_(22) cancer cells by 2.3 and 3.7-fold, and the mitochondrial Ca 2+ /Mg 2+ -ATPase activity decreased by 28%. And 58%, ATP concentration increased T 77% and 83%. The results suggest that Wuhong ink may reduce the intracellular Ca~(2+) concentration, and subsequently affect the mitochondrial Ca~(2+)-dependent Ca~(2+)/Mg~(2+)-ATPase activity and reduce Ca~( The transport of 2+) into mitochondria reduces or even eliminates the inhibitory effect of Ca 2+ on ATP synthesis. This may be one of the mechanisms by which squid ink inhibits tumor growth. rrrrrrrrrn