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以红丰11为轮回亲本、Clark为供体亲本构建回交群体进行耐旱性鉴定,并对获得的选择群体进行全基因组SSR标记扫描,计算供体基因型导入频率,同时利用卡方测验检测偏分离SSR位点,对株高进行QTL定位。结果表明:卡方测验检测到11个SSR偏分离位点(超导入)分布于8条连锁群;株高共定位8个QTL位点分布于A1、C2、E、F、L、M和O连锁群。在C2连锁群的Satt305位点,耐旱供体等位基因显著性检测和株高QTL位点具有一致性;在E连锁群的Sat_136位点,F连锁群的Satt586和GMRUBP位点,L连锁群的Satt156位点,M连锁群的GMSL514位点均与有一致性,这些位点可能是控制大豆耐旱性的重要位点。
Using Hongfeng 11 as the reincarnation parent, Clark constructed the backcross population for donor parents to identify their drought tolerance. Genome-wide SSR markers were screened among the selected populations, and the frequency of donor genotypes was calculated. At the same time, chi-square test Partial SSR segregation, QTL mapping of plant height. The results showed that 11 SSR segregation sites (superconductors) were detected in 8 linkage groups by chi-square test. Eight QTLs were co-located in A1, C2, E, F, L, M and O Chain group. At the Satt305 locus of C2 linkage group, the alleles of drought-tolerant donor were consistent with those of QTL loci at the Sat_136 locus of E linkage group, Satt586 and GMRUBP loci of F linkage group, L linkage The Satt156 locus in the population and the GMSL514 locus in M linkage group are consistent with each other, and these loci may be important sites for controlling the drought tolerance of soybean.