论文部分内容阅读
Introduction Single nucleotide polymorphisms(SNPs) are themost abundant DNA markers in the human genome oc-curring at a frequency of one in every 500—1000 nu-cleotides[1]. A variety of methods have been used forthe analysis of single nucleotide polymorphisms, inclu-ding restriction fragment length polymorphism(RFLP)[2], direct sequencing by using laser-inducedfluorescence detection[3], fluorescence energy trans-fer[4], MALDI-TOF MS combined with primer exten-sion or invasive cleavage[5,6], and fluorescence polari-zation[7]. During the past two decades, mass spectro-metry has become a very popular tool in the analysis ofbiomolecules and is perfectly suited to the analysis ofsingle nucleotide polymorphisms ( SNPs) due to itsspeed, lowcost, and accuracy.
Introduction Single nucleotide polymorphisms (SNPs) are themost abundant DNA markers in the human genome oc-curring at a frequency of one in every 500-1000 nu-cleotides [1]. A variety of methods have been used forthe analysis of single nucleotide polymorphisms, includs dlf restriction fragment length polymorphism (RFLP) [2], direct sequencing by using laser-induced fluorescence detection [3], fluorescence energy trans-fer [4], MALDI-TOF MS combined with primer extenion or invasive cleavage [5 , 6], and fluorescence polari-zation [7]. During the past two decades, mass spectro-metry has become very popular tool in the analysis of biomolecules and is perfectly suited to the analysis of single nucleotide polymorphisms (SNPs) due to itsspeed, lowcost, and accuracy.