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目的对广东省中山市2007年-2012年间分离的两株W135群脑膜炎奈瑟菌进行病原分子特征分析,调查两者之间的关系,并探索其可能来源。方法使用16S rRNA基因分型、porA可变区分型及多位点序列分型(multilocus sequence typing,MLST)对两株W135群脑膜炎奈瑟菌(ZS07株,ZS12株)进行分子生物学分析。结果两株W135群脑膜炎奈瑟菌分型结果一致,16S rRNA分型为Group 31,MLST型别为ST-11/ET-37(等位基因谱:2,3,4,3,8,4,6),porA测序分型测序为:P1.5,2。结论脑膜炎奈瑟菌ZS07株与ZS12株的亲缘关系高度同源,与我国近几年流行的脑膜炎奈瑟菌亲缘关系较远,与2000年麦加朝圣后由朝觐者引起全球流脑流行的W135群菌株关系密切。
Objective To analyze the molecular characteristics of two strains of Neisseria meningitidis from W135 isolated from 2007 to 2012 in Zhongshan City, Guangdong Province, and to investigate the possible relationship between them. Methods Two strains of Neisseria meningitidis W135 (ZS07 and ZS12) were analyzed by 16S rRNA genotyping, porA mutagenesis and multilocus sequence typing (MLST). Results The results showed that the genotypes of Neisseria meningitidis in the two W135 strains were identical, the genotypes of 16S rRNA were Group 31, and the genotype of MLST was ST-11 / ET-37 (the allelic frequency was 2, 3, 4,6), porA sequencing typing sequencing: P1.5,2. Conclusion Neisseria meningitidis strain ZS07 is highly homologous to ZS12 strain, and has a close relationship with Neisseria meningitidis epidemic in China in recent years. After the pilgrimage to Mecca in 2000, the global meningitis caused by pilgrims W135 group of strains closely related.