论文部分内容阅读
目的:观察肾康丸对糖尿病(DM)大鼠模型的肾功能及Ⅰ型胶原合成的影响。方法:用链脲佐菌素(STZ)腹腔注射法诱导建立大鼠DM模型,模型成功后随机分为模型对照组、开搏通组、肾康丸组3组,另设正常对照组。治疗8周,观察治疗期间及治疗后大鼠一般状况、血糖、24h尿蛋白、肌酐、尿素氮、肾质量、相对肾质量,并用实时荧光定量PCR测定肾脏Ⅰ型胶原(collagenⅠ)和转化生长因子-β1(TGF-β1)mRNA的表达情况。结果:造模3组均出现DM及肾脏损害,肾脏collagenⅠ、TGF-β1mRNA的表达明显增加。肾康丸和开搏通均可改善大鼠基本状况、肾功能、尿蛋白,下调肾脏组织collagenⅠ、TGF-β1mRNA的表达;肾康丸组血糖浓度显著降低(P<0.01),其降血糖疗效优于开搏通(P<0.01)。结论:肾康丸可以减少col-lagenⅠ、TGF-β1的产生,延缓糖尿病肾病进程。
OBJECTIVE: To observe the effect of Shenkangwan on renal function and type I collagen synthesis in a diabetic (DM) rat model. METHODS: Rat DM model was induced by intraperitoneal injection of streptozotocin (STZ). After the model was successfully established, it was randomly divided into three groups: model control group, captopril group, and Shenkangwan group. Another normal control group was established. After 8 weeks of treatment, the general state of rats, blood glucose, 24h urinary protein, creatinine, urea nitrogen, kidney mass, and relative kidney mass were observed during and after treatment. Collagen I and transforming growth factor were measured by real-time fluorescence quantitative PCR. -β1 (TGF-β1) mRNA expression. RESULTS: DM and renal lesions were observed in all three groups, and the expression of collagenI and TGF-β1 mRNA in kidneys increased significantly. Both Shenkang Pills and Captopril can improve the basic condition, renal function and urinary protein of rats, and down-regulate the expression of collagen I and TGF-β1 mRNA in kidney tissue. The concentration of blood glucose in Shenkang Pill group is significantly reduced (P<0.01), and its hypoglycemic effect is excellent. In the cappuccino (P <0.01). Conclusion: Shenkangwan can reduce the production of col-lagen I and TGF-β1 and delay the progression of diabetic nephropathy.