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脑胶质瘤是目前难治的恶性肿瘤之一,寻找有效的效应细胞是目前研究的方向.我们根据文献报道的方法,用自己研制的瘤苗,给昆明种小鼠体内致敏,取其脾细胞,体外再次用胶质瘤细胞刺激,获得特异性异源性CTL.具体方法是用榄香烯和丝裂霉素共同处理等量G422胶质瘤培养18小时,可得胶质瘤特异性瘤苗(榄香烯/丝裂霉素瘤苗),配制成单细胞悬液2×10~6/ml.取上述瘤苗1ml给昆明鼠多点注射体内致敬,2周后重复一次.第二次致敏10天后,取其脾细胞.在RPMI1640培养液1ml中加入脾细胞2×10~6、G422瘤细胞4×10~5和rIL-2400U,共同培养10天,收获特异性源性CTL.分别做正常脾细胞、体外经rIL-2培养的正常脾细胞(LAK)、
Glioma is currently one of the refractory malignant tumors, looking for effective effector cells is the direction of the current research.We reported the method according to the literature, with their own vaccine developed to Kunming mice sensitized, whichever is Spleen cells in vitro stimulation with glioma cells again to obtain specific allogenic CTL specific method is elemene and mitomycin co-treatment of an equal amount of G422 glioma cultured for 18 hours can be glioma-specific Tumor vaccine (elemene / mitomycin tumor vaccine), prepared into single cell suspension 2 × 10 ~ 6 / ml take the tumor vaccine 1ml to Kunming mice injected more in vivo, two weeks after the repeat. After the second sensitization for 10 days, the splenocytes were collected, spleen cells (2 × 10 ~ 6), G422 tumor cells (4 × 10 ~ 5) and rIL-2400U were added into 1ml of RPMI1640 culture medium for co-culture for 10 days, CTL were made normal spleen cells in vitro by rIL-2 cultured normal spleen cells (LAK)