论文部分内容阅读
目的探究湖北省当阳地区女性宫颈人乳头瘤病毒(HPV)感染及流行病学调查情况,为宫颈癌的早期筛查与预防建立理论根据。方法选取2013年1-12月在湖北省当阳地区2 564例宫颈脱落细胞标本,按年龄段分组,利用以PCR体外扩增和DNA反向点杂交为基础的DNA芯片技术,对样本进行HPV检测并加以分型,统计各组HPV感染阳性率、基因型及亚型分布情况、多重感染情况。结果 2 564例妇女中HPV感染阳性518例,阳性率20.20%,21~25岁和51~55岁两个年龄段明显高于其他年龄段,差异有统计学意义(P<0.05);共检测出包括低危型的HPV6、11、43、44和高危型的HPV16、18、31、33、35、39、45、51、52、56、58、59、68、73、82、83在内的20种基因型,其中低危型中HPV43比例最高,高危型中HPV16比例最高;单一感染400例,占77.22%;双重感染78例,占15.06%;三重感染31例,占5.98%,四重感染9例,占1.74%。单纯低危型感染103例,占19.88%;单纯高危型感染359例,占69.31%;低危型与高危型同时感染56例(10.81%),随着年龄增长高危型感染的构成比不断升高。结论针对不同年龄段女性进行宫颈HPV感染情况及流行病学的调查分析为宫颈癌的预防、早期诊断及治疗建立了理论根据。
Objective To investigate the prevalence and epidemiological investigation of human cervical papillomavirus (HPV) in Dangyang, Hubei Province, and to establish a theoretical basis for early screening and prevention of cervical cancer. Methods From January to December in 2013, 2 564 cervical exfoliated cells in Dangyang district of Hubei province were selected and divided into groups according to their ages. The DNA microarray technique based on PCR in vitro amplification and DNA reverse hybridization was used to detect HPV Detection and classification, statistical HPV infection in each group the positive rate, genotype and subtype distribution, multiple infections. Results The positive rate of HPV infection in 2 564 women was 518 cases, the positive rate was 20.20%. The two age groups 21- 25 and 51-55 were significantly higher than other age groups (P <0.05) Including low-risk HPV6,11,43,44 and high-risk HPV16,18,31,33,35,39,45,51,52,56,58,59,68,73,82,83 Of the 20 genotypes, of which HPV43 was the highest in low-risk type and highest in high-risk type; 400 cases were single infection, accounting for 77.22%; 78 cases were double-infection, accounting for 15.06%; 31 cases were triple infection, accounting for 5.98% Severe infection in 9 cases, accounting for 1.74%. The simple low-risk infection in 103 cases, accounting for 19.88%; 359 cases of pure high-risk infection, accounting for 69.31%; low-risk type and high-risk type 56 cases (10.81%), with age, the proportion of high- high. Conclusions The investigation and analysis of cervical HPV infection in different age groups and epidemiology of women have established the theoretical basis for the prevention, early diagnosis and treatment of cervical cancer.