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目的 探讨消化道恶性肿瘤细胞中三联脆组 (fragilehistidinetriad ,FHIT)基因的异常转录情况。方法 应用巢式逆转录聚合酶链反应 (nestedRT PCR)对 96例消化道恶性肿瘤 ( 2 1例食管癌、4 3例胃癌和 3 2例结肠癌 )及相应的癌旁组织 ,18例正常组织FHIT基因cDNA片断进行扩增。结果 在3 3 .3 %的食管癌组织 ,5 1.2 %的胃癌组织和 3 1.3 %的结肠癌组织中检出异常FHIT转录 ,而在相应的食管、胃、结肠癌旁组织中FHIT异常转录检出率分别为 4 .8%、2 0 .9%和 9.4 % ,二者差异有显著性 (P <0 .0 5 ) ;18例正常组织中未见FHIT异常转录。结论 食管癌、胃癌和结肠癌组织FHIT基因的异常转录可能与消化道恶性肿瘤的发生、发展有关。
Objective To investigate the abnormal transcription of the fragilehistidinetriad (FHIT) gene in malignant cells of the digestive tract. Methods Nested RT PCR was used to analyze 96 cases of malignant tumors of the digestive tract (21 cases of esophageal cancer, 43 cases of gastric cancer, and 32 cases of colon cancer) and the corresponding adjacent tissues, and 18 cases of normal tissues. The FHIT gene cDNA fragment was amplified. RESULTS: Abnormal FHIT transcription was detected in 33.3% of esophageal cancer tissues, 51.2% of gastric cancer tissues, and 31.3% of colorectal cancer tissues. FHIT abnormal transcription was detected in the corresponding esophageal, gastric, and colon cancer tissues. The rates were 4.8%, 20.9%, and 9.4%, respectively. There was a significant difference between the two groups (P < 0.05). No abnormal transcription of FHIT was found in 18 normal tissues. Conclusion The abnormal transcription of FHIT gene in esophageal cancer, gastric cancer and colon cancer may be related to the occurrence and development of digestive tract malignancy.