以芦丁为对照品,采用分光光度法测定了长白山区野生金灯藤成熟种子及种皮中总黄酮含量。芦丁在8.16—48.96μg/mL的范围内呈良好线性关系,测得种子及种皮中总黄酮含量分别为2.71%、0.91%,平均加标回收率为99.32%。该方法简单可行,灵敏度高,测定结果可靠,可用于测定金灯藤种子及种皮中总黄酮含量。 Using rutin as reference substance, the content of total flavonoids in the mature seeds and seed coat of wild Auchanla giganteum in Changbai Mountains was determined by spectrophotometry. Rutin showed a good linear relationship in the range of 8.16-48.96μg / mL. The content of total flavonoids in seeds and seed coat was 2.71% and 0.91%, respectively. The average recovery was 99.32%. The method is simple and feasible, high sensitivity and reliable determination results, and can be used for determining the content of total flavonoids in seed and seed coat of golden lamputin.