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AIM To assess the effect of sodium selenite on the severity of dextran sulfate sodium(DSS)-induced colitis in C57BL/6 mice.METHODS Mice were randomly divided into four groups(n = 10/group): normal group, selenium(Se) group, chronic colitis group, and Se + chronic colitis group. The mice were sacrificed on day 26. Survival rates, clinical symptoms, colon length, and histological changes were determined. The percentages and absolute numbers of immune system cells in the lamina propria lymphocytes(LPL) of the colon, the expression of m RNA in colon tissue, and the concentrations of Th1, Th17, and Treg cytokines in LPL from the large intestine, were measured.RESULTS Se significantly ameliorated the symptoms of colitis and histological injury(P < 0.05 each), increasing the proportions of neutrophils and CD4+ CD25+ T cells(P < 0.05 each) and decreasing the proportions of γδT cells, CD4+, CD4+CD44+, and CD4+ CD69+ T cells in LPL(P < 0.05 each). Moreover, Se reduced the expression of IL-6, IFN-γ, IL-17 A, IL-21, T-bet, and RORγt(P < 0.05 each), but enhanced the expression of IL-10 and Foxp3(P < 0.05 each). CONCLUSION These results suggest that Se protects against DSSinduced chronic colitis perhaps by increasing the number of CD4(+)CD25(+) Tregs that suppress the secretion of proinflammatory cytokines and populations of Th1, Th17, and γδT cells.
AIM To assess the effect of sodium selenite on the severity of dextran sulfate sodium (DSS) -induced colitis in C57BL / 6 mice. METHODS Mice were differentiated into four groups (n = 10 / group): normal group, selenium (Se) group, chronic colitis group, and Se + chronic colitis group. The mice were sacrificed on day 26. Survival rates, clinical symptoms, colon length, and histological changes were determined. The percentages and absolute numbers of immune system cells in the lamina propria lymphocytes (LPL) of the colon, the expression of m RNA in colon tissue, and the concentrations of Th1, Th17, and Treg cytokines in LPL from the large intestine, were measured .RESULTS Sected a markedly symptoms of colitis and histological injury (P <0.05 each), increasing proportions of neutrophils and CD4 + CD25 + T cells (P <0.05 each) and decreasing the proportions of γδ T cells, CD4 +, CD4 + CD44 +, and CD4 + CD69 + T cells in LPL , Se reduced the expression of IL-6, I. CONCLUSION These results suggest that Se protects (P <0.05 each), FN-γ, IL-17 A, IL-21, T-bet, and RORγt (P <0.05 each) but enhanced the expression of IL-10 and Foxp3 against DSSinduced chronic colitis may by increasing the number of CD4 (+) CD25 (+) Tregs that suppress the secretion of proinflammatory cytokines and populations of Th1, Th17, and γδ T cells.