论文部分内容阅读
红三叶草粉末经70%乙醇室温提取、43℃减压浓缩,过大孔吸附树脂柱,再先后用蒸馏水和70%乙醇洗脱,收集70%乙醇洗脱液,减压浓缩得红三叶草醇提物。为了提高红三叶草醇提物体外抗氧化活性,将红三叶草醇提物与纤维素酶在50℃恒温条件下反应30min,比较酶解前后红三叶草醇提物的清除DPPH自由基能力和还原力。结果表明:在相同的浓度下,经纤维素酶处理后的红三叶草醇提物抗氧化能力要高于未被处理的红三叶草醇提物。
The red clover powder was extracted by 70% ethanol at room temperature, concentrated under reduced pressure at 43 ℃, passed through a macroporous resin column, eluted with distilled water and then with 70% ethanol, collected 70% ethanol eluate and concentrated under reduced pressure to obtain red clover alcohol extract Things. In order to improve the antioxidant activity of red clover alcohol extract in vitro, red clover alcohol extract was reacted with cellulase at 50 ℃ for 30 min. The DPPH radical scavenging ability and reducing power of red clover alcohol extract before and after enzymolysis were compared. The results showed that at the same concentration, cellulosic red clover alcohol extract antioxidant capacity is higher than the untreated red clover alcohol extract.