论文部分内容阅读
目的比较含有一定浓度的蓖麻籽提取物蓖麻毒蛋白对小白鼠生殖功能的影响,为配制植物源灭鼠剂提供理论基础。方法给健康性成熟雌雄小白鼠分别食用拌有蓖麻籽提取物的混合饵料,计算适口性,睾丸、卵巢脏器系数;制作睾丸、卵巢组织学切片,统计合笼后的产仔情况。结果食用蓖麻籽提取物混合饵料的实验组小白鼠适口性与对照组比较,差异无统计学意义(雄:t=1.360,P=0.227;雌:t=1.170,P=0.449);实验组雄鼠睾丸脏器系数为4.118×10-3,与对照组(7.398×10-3)比较系数减小,且差异有统计学意义(t=2.740,P=0.013);实验组生殖上皮厚度为(26.55±6.11)μm,与对照组〔(54.35±7.14)μm〕比较厚度变薄,且差异有统计学意义(t=3.160,P=0.011)。实验组1产仔潜伏期为(7.50±0.71)d,产仔数量为(3.80±5.22)只,实验组3产仔潜伏期为(10.00±0.00)d,产仔数量为(0.40±0.89)只,对照组产仔潜伏期为(2.20±1.30)d,产仔数量为(13.50±2.97)只,实验组1和3与对照组比较产仔潜伏期延长(Z=1.954,P=0.049;Z=3.485,P=0.002),产仔数量减少(Z=2.550,P=0.011;Z=3.424,P=0.004),两者差异均有统计学意义。实验组雌性卵巢脏器系数为2.218×10-3,与对照组(4.621×10-3)比较脏器系数减小,且差异有统计学意义(t=3.980,P=0.001)。实验组黄体最大横截面积为(12 347.50±2 359.28)μm2,与对照组〔(19 625.47±6 612.40)μm2〕比较横截面积减小,且差异有统计学意义(t=2.750,P=0.013)。实验组2产仔潜伏期为(12.00±0.00)d,产仔数量为(1.80±4.02)只,与对照组比较产仔潜伏期延长(Z=4.485,P=0.001),产仔数量减少(Z=2.703,P=0.007),两者差异均有统计学意义。结论蓖麻毒蛋白配制的混合饵料对小白鼠的抗生育效果明显且适口性好。作为绿色环保型鼠药应用到农田、林地还有待于进一步研究。
Objective To compare the effects of castor bean extract ricin with a certain concentration on the reproductive function of mice and to provide a theoretical basis for the preparation of rodenticides. Methods The healthy mature male and female mice were fed with mixed feed mixed with castor bean extract to calculate the coefficient of palatability, testis and ovary organ. The testes and ovarian histological sections were made and the litter size was calculated. Results There was no significant difference in the palatability between the experimental group and the control group (r = 1.360, P = 0.227; females: t = 1.170, P = 0.449) The coefficient of testis in male rats was 4.118 × 10-3, which was significantly lower than that in control group (7.398 × 10-3) (t = 2.740, P = 0.013). The thickness of reproductive epithelium in experimental group was (26.55 ± 6.11) μm, compared with the control group 〔(54.35 ± 7.14) μm〕 thinning, and the difference was statistically significant (t = 3.160, P = 0.011). Litter incubation period was (7.50 ± 0.71) days and the number of litter size was (3.80 ± 5.22) days in experiment group 1, (10.00 ± 0.00) d and (0.40 ± 0.89) days in experimental group 3, The litter size was (2.20 ± 1.30) days in the control group and (13.50 ± 2.97) days in the control group, and the latency of the litter size in the experimental group 1 and 3 was longer than that in the control group (Z = 1.954, P = 0.049; P = 0.002). The number of litter size decreased (Z = 2.550, P = 0.011; Z = 3.424, P = 0.004). There was significant difference between the two groups. The ovarial organ coefficient of the experimental group was 2.218 × 10-3, which was significantly lower than that of the control group (4.621 × 10-3), and the difference was statistically significant (t = 3.980, P = 0.001). The largest cross-sectional area of the corpus luteum in the experimental group was (12 347.50 ± 2 359.28) μm 2, which was significantly lower than that in the control group [(19 625.47 ± 6 612.40) μm 2〕 (t = 2.750, P = 0.013). Litter latency was (12.00 ± 0.00) d and the number of litter size was (1.80 ± 4.02) in experimental group 2, and the litter size was prolonged (Z = 4.485, P = 0.001) 2.703, P = 0.007), both differences were statistically significant. Conclusion The mixed feedstuffs prepared by ricin have obvious antibacterial effect on mice and good palatability. As a green rat poison applied to farmland, woodland remains to be further studied.