Dear Editor,Haessing genetic diversity and the introduction of elite alleles from wild relatives or landraces into commercial cultivars has been a major goal in crop breeding programs.Precise modification of the plant genome through clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas)-mediated homology-directed repair (HDR) offers a great promise to introduce elite alleles from wild relatives or landraces into commercialized cultivars in the short term.Various strategies have been attempted for precise targeted gene/allele replacement or gene insertion and tagging through CRISPR/Cas systems in plants (Baltes et al., 2014;GilHumanes et al., 2016;Sun et al., 2016;Wang et al., 2017;Li et al., 2019;Miki et al., 2018;Hua et al., 2019;Wolter and Puchta, 2019;Dong et al., 2020).However, due to the intrinsic lower frequency of HDR in plant cells, insufficient availability of a donor repair template, and the non-homologous end-joining (NHEJ) is the predominant DNA repair pathway in plants (Baltes et al., 2014;Li et al., 2019), it remains challenging, especially in crop plants.Thus, it is essential to further exploit more efficient precision genome-editing technology in order to accelerate crop improvement.