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A reversed phase high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 12,13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.A Grace Apollo C 18 column (250 mm 4.6 mm,5 mm) was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid (0.2%,v/v).Gradient elution was carried out at the flow rate of 1.0 mL/min and the column temperature was 30 1C.An ultraviolet (UV) detector was used with a selected wavelength of 240 nm.Calibration curves were linear within the concentration range of 4.6-45.75 mg/mL for 12,13-dihydroxyeuparin (r40.9999) and 106.9-1068.9 mg/mL for glycyrrhizic acid (r40.9999),respectively.Recoveries were 102.18% for 12,13-dihydroxyeuparin and 101.17% for glycyrrhizic acid.The method developed could be applied to the simultaneous determination of 12,13dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.
A reversed phase high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 12,13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo C 18 column (250 mm 4.6 mm, 5 mm) was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid (0.2%, v / v) .Gradient elution was carried out at the flow rate of 1.0 mL / min and the column temperature was 30 1C. An ultraviolet (UV) detector was used with a selected wavelength of 240 nm. Calibration curves were linear within the concentration range of 4.6-45.75 mg / mL for 12,13-dihydroxyeuparin (r40.9999) and 106.9-1068.9 mg / mL for glycyrrhizic acid (r40.9999 ), respectively. Recoveries were 102.18% for 12,13-dihydroxyeuparin and 101.17% for glycyrrhizic acid. The method developed could be applied to the simultaneous determination of 12,13 dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.