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为了研究SLE的T、B淋巴细胞及其亚类的凋亡情况,采用碘化丙锭染色,在流式细胞仪下观察计数:22例SLE病人淋巴细胞(包括总体淋巴细胞、CD3~+、CD4~+、CD8~+T细胞和CDl9~+B细胞)凋亡率在培养0、24、48h时均较正常组有显著增高,并以CD4~+T细胞和CDl9~+B细胞在活动期SLE病人中凋亡更突出。此外,SLE病人,自身抗体产生愈多,其细胞凋亡率愈高;疾病活动度增高,凋亡率也较高。提示;SLE病人的淋巴细胞凋亡在体外加速,与其自身抗体产生有密切关系,在其发病机制中起一定的作用。
In order to study the apoptosis of SLE, B lymphocytes and their subclasses in SLE, counting with propidium iodide and counting by flow cytometry: 22 cases of SLE patients with lymphocytes (including total lymphocytes, CD3 + CD4 ~ +, CD8 ~ + T cells and CD19 ~ + B cells) were significantly increased at 0, 24 and 48h, and CD4 ~ + T cells and CD19 ~ + B cells Apoptosis is more prominent in patients with SLE. In addition, SLE patients, autoantibodies produce more, the higher the rate of apoptosis; disease activity increased, the apoptosis rate is also higher. Tip; SLE patients with lymphocyte apoptosis accelerated in vitro, and its autoantibodies are closely related to its pathogenesis play a role.