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试验于2000年在东北农业大学院内试验田进行,从大豆正茬、迎茬、连作、7年连作(自1994年连续种植大豆)田间土壤中分离出优势真菌进行实验。研究表明,土壤真菌优势类群在苗期以木霉、青霉、镰刀菌为主,在盛花期和结荚期以青霉、毛霉、黑根霉为主,连作大豆的土壤真菌优势类群的种类、数量高于其它茬口。经不同茬口土壤真菌毒素液处理大豆种子后,表现出抑制大豆种子发芽,各处理间由于品种不同、茬口不同略有差别,抑制作用为连作>迎茬>正茬,7年连作处理的抑制作用有强有弱。
The experiment was carried out in the experimental farm of Northeast Agricultural University in 2000, and the experiment was carried out by separating the dominant fungi from the soil of soybean continuous cropping, continuous cropping, continuous cropping and continuous cropping for seven years since 1994. The results showed that the dominant groups of soil fungi were Trichoderma, Penicillium and Fusarium at the seedling stage, and Penicillium, Mucor and Rhizopus were dominant during the flowering and podding stages. Type, quantity is higher than other stubble. Soybean seeds were treated with mycotoxins from different stubble sections and showed inhibition of germination of soybean seeds. Because of different varieties and stubble, there was a slight difference between the treatments, and the inhibitory effect was continuous cropping> continuous cropping> 7-year continuous cropping inhibition Strong and weak.