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目的以獐牙菜苦苷为底物,用黑曲霉菌对獐牙菜苦苷进行生物转化,并对转化条件进行优化。方法以獐牙菜新素生成率和獐牙菜苦苷转化率为指标,考察不同培养时间、不同碳源、不同氮源、不同种类金属离子、磷酸盐、生长因子(酵母膏)、接种量、底物加入量、不同初始pH值、不同温度条件,优化獐牙菜苦苷在黑曲霉培养液中的转化条件。结果优化后的培养条件为:葡萄糖10 g/L,蛋白胨5 g/L,酵母膏5 g/L,KH2PO4 5 g/L,MgSO4 1 g/L,CaCl2 1 g/L,初始pH 6.0,接种量0.5%,底物加入量1 mg/mL,28℃,培养5 d。结论黑曲霉转化獐牙菜苦苷生成獐牙菜新素的生成率稳定在8%左右。
Objective To purify swertia glycosides with swertia glycoside as a substrate, and to optimize the transformation conditions. Methods The production rate of swertiain and the conversion of swertia were used as indexes to investigate the effects of different culture time, different carbon sources, different nitrogen sources, different kinds of metal ions, phosphate, growth factor (yeast extract), inoculum size , Substrate addition amount, different initial pH value and different temperature conditions, the conditions of swertia glycoside transformation in Aspergillus niger culture medium were optimized. Results The optimized culture conditions were: glucose 10 g / L, peptone 5 g / L, yeast extract 5 g / L, KH2PO4 5 g / L, MgSO4 1 g / L, CaCl2 1 g / Amount of 0.5%, substrate added 1 mg / mL, 28 ℃, cultured for 5 d. Conclusion Aspergillus niger transformed swertia glycoside formation of swertin new factor formation rate stabilized at about 8%.