论文部分内容阅读
目的:研究三甲氧基二苯乙烯与顺铂对人胃癌BGC823细胞的抑制作用。方法:设空白对照组、顺铂治疗组、三甲氧基二苯乙烯治疗组、顺铂及三甲氧基二苯乙烯联合治疗组。用MTT法测定顺铂和三甲氧基二苯乙烯对细胞的抑制作用;用电镜、流式细胞仪观察其细胞变化。结果:顺铂与三甲氧基二苯乙烯合用,细胞存活率较单用时明显降低。顺铂100μg/L细胞存活率为(67.23±8.91)%,三甲氧基二苯乙烯100μg/L细胞存活率为(51.42±9.56)%;细胞存活率在合并用药时细胞存活率明显下降,顺铂10μg/L和三甲氧基二苯乙烯10μg/L的细胞存活率为(43.62±8.34)%,浓度150μg/L时细胞存活率最低为(17.33±7.93)%。联合用药后,顺铂使用量的IC50是(110.0±9.8)μg/L,三甲氧基二苯乙烯使用量的IC50是(18.0±10.0)μg/L,增效倍数分别为2.51、4.03合并指数CI50是0.58,CI50<0.95。细胞生长多停滞在S期(58.0±4.0)%。结论:顺铂对胃癌BGC823细胞有抑制作用,少量的顺铂与三甲氧基二苯乙烯合用后可达到大剂量顺铂单药化疗的效果,产生了协同作用。
Objective: To study the inhibitory effect of trimethoxy-stilbene and cisplatin on human gastric cancer BGC823 cells. Methods: The blank control group, cisplatin treatment group, trimethoxy stilbene treatment group, cisplatin and trimethoxy stilbene combination treatment group. The inhibitory effect of cisplatin and trimethoxy-stilbene on cells was determined by MTT assay. The changes of cells were observed by electron microscopy and flow cytometry. Results: The combination of cisplatin and trimethoxy-stilbene decreased the cell viability significantly compared with the single use. The cell viability of 100μg / L cisplatin was (67.23 ± 8.91)% and that of trimethoxystilbene 100μg / L was (51.42 ± 9.56)%. The cell survival rate of cisplatin was significantly decreased when combined with cisplatin The cell survival rate of platinum 10μg / L and trimethoxy stilbene 10μg / L was (43.62 ± 8.34)%, and the lowest cell survival rate was (17.33 ± 7.93)% at the concentration of 150μg / L. After combined treatment, the IC50 of cisplatin was (110.0 ± 9.8) μg / L, the IC50 of trimethoxy stilbene was (18.0 ± 10.0) μg / L, and the synergistic multiples were 2.51 and 4.03, respectively CI50 is 0.58 and CI50 <0.95. More cell growth arrest in the S phase (58.0 ± 4.0)%. CONCLUSION: Cisplatin inhibits gastric cancer BGC823 cells. A small amount of cisplatin combined with trimethoxy-stilbene can achieve the effect of high-dose cisplatin single-agent chemotherapy and produce synergistic effect.