论文部分内容阅读
对中国南海500 m深海水来源真菌Exophiala sp.SCSIO 05791进行了次级代谢产物研究;综合运用硅胶柱层析、Sephadex LH-20凝胶柱层析、中压反相柱层析以及半制备高效液相等色谱学方法对其大米发酵产物进行分离纯化,从中分离到8个单体化合物,利用核磁共振、质谱等波谱学手段并结合其理化性质及文献数据鉴定了其化学结构:stephacidin A(1)、吲哚-3-乙酸(2)、环(D)-脯氨酸-(D)-苯丙氨酸(3)、反式阿魏酸(4)、顺式阿魏酸(5)、2-(乙酰氨基)-苯酚(6)、氨基苯甲酸(7)、4-羟基苯酞(8)。化合物1~8均为首次从该属中分离得到。
The secondary metabolites of the fungus Exophiala sp. SCSIO 05791, a 500-m deep-seawater source in the South China Sea, were studied. Silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure reversed-phase column chromatography and semi-preparative high performance Liquid chromatography (HPLC) was used to separate and purify the rice fermentation products. Eight monomer compounds were isolated from them. Their chemical structures were identified by NMR, MS and other spectroscopic methods. The chemical structure of stephacidin A ( (D) -proline- (D) -phenylalanine (3), trans-ferulic acid (4), cis-ferulic acid ), 2- (acetylamino) -phenol (6), aminobenzoic acid (7) and 4-hydroxyphthalide (8). Compounds 1 to 8 were isolated from this genus for the first time.