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为探讨旋毛虫ES抗原对RAW264.7细胞TLR2/4mRNA表达的影响,分别取经0、2、5、15、30、45μg/mL ES抗原作用24h的RAW264.7细胞和用15μg/mL ES抗原作用0、3、6、12、18、24h后的RAW264.7细胞,采用半定量PCR方法检测TLR2和TLR4mRNA的表达水平变化。结果显示,随着ES抗原浓度的升高,TLR2/4mRNA的表达量逐渐上升,15μg/mL ES抗原组与空白对照组相比差异显著(P<0.05)。在15μg/mL ES抗原作用24h内,随着作用时间的延长,TLR2/4mRNA的表达量逐渐上升,作用18h后的表达水平升高,且与空白对照组差异显著(P<0.05)。证实,ES抗原可刺激RAW264.7细胞表面受体TLR2/4表达升高,且存在一定的剂量和时间效应。
To investigate the effect of Trichinella ES antigen on TLR2 / 4 mRNA expression in RAW264.7 cells, RAW264.7 cells treated with 0, 2, 5, 15, 30 and 45 μg / mL ES antigen for 24 h and 15 μg / mL ES antigen The expression of TLR2 and TLR4 mRNA in RAW264.7 cells were detected by semi-quantitative PCR at 0, 3, 6, 12, 18 and 24 h after transfection. The results showed that with the increase of ES antigen concentration, the expression of TLR2 / 4 mRNA gradually increased, and the difference was significant (P <0.05) between 15μg / mL ES antigen group and blank control group. The expression of TLR2 / 4 mRNA gradually increased after treated with 15μg / mL ES antigen for 24 hours, and the expression of TLR2 / 4 mRNA increased gradually after 18 hours of treatment, which was significantly different from that of the blank control group (P <0.05). Confirmed that ES antigen can stimulate RAW264.7 cell surface receptor TLR2 / 4 expression increased, and there is a certain dose and time effect.