日本研究人员采用实时定量逆转录聚合酶链反应(qRT-PCR),分析了CLDN4 mRNA在一组共9个胰腺癌细胞系和取自100例PDAC患者的福尔马林固定石蜡包埋(FFPE)组织中的表达情况,并与临床病理状况和患者预后进行关联分析。研究者还采用免疫组化法在20个PDAC FFPE标本中分析了CLDN4蛋白表达情况。结果显示,与正常胰腺导管细胞和成纤维细胞相比,所有被检测的胰腺癌细胞系CLDN4表达水平升高。研 Japanese researchers used quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) to analyze CLDN4 mRNA expression in a panel of 9 pancreatic cancer cell lines and formalin-fixed paraffin-embedded (FFPE ) Tissue expression, and with the clinical and pathological status and prognosis of patients associated analysis. The researchers also used immunohistochemistry in 20 PDAC FFPE specimens analyzed CLDN4 protein expression. The results showed that compared with normal pancreatic ductal cells and fibroblasts, all tested pancreatic cancer cell lines CLDN4 expression levels increased. research