论文部分内容阅读
目的探讨急性肾损伤早期肾实质血流灌注的变化规律。方法将40只雄性新西兰大白兔随机分为5组,每组8只,经耳缘静脉注射顺铂建立急性肾损伤模型,于造模后6、12、24、36h及48h进行双肾超声造影检查及测血SCr,分析不同时间点造影动态图像,获取TIC曲线及相关参数。以SCr较基础值升高1.5倍或以上为AKI诊断标准,使用SPSS 22.0及Medcalc对数据进行分析,以P<0.05为差异具有统计学意义。取兔双肾做常规石蜡切片,HE染色光镜下观察。结果 T24、T36组及T48组实验兔均发生了AKI。与建模前相比,建模后T12、T24、T36组及T48组皮质RT,T24、T36组及T48组皮质PI、T_(1/2)、mTT及TTP,T36组及T48组皮质AUC及WIS均发生了显著改变(P<0.05),髓质各造影参数均未发生显著改变。部分造影指标对AKI诊断效能较佳,灵敏度较高。结论超声造影可良好的反映AKI早期肾皮质血流灌注的改变,皮质RT可较SCr早期发现AKI,且具有较好的诊断效能。
Objective To investigate the changes of early renal parenchymal perfusion in acute renal injury. Methods Forty male New Zealand white rabbits were randomly divided into five groups (n = 8 each). Acute kidney injury model was established by injecting cisplatin into the ear vein, and bilateral renal contrast echocardiography was performed at 6, 12, 24, 36 h and 48 h after modeling Check and blood test SCr, analysis of dynamic images at different time points to obtain TIC curve and related parameters. To SCr 1.5 times higher than the baseline value or more for the AKI diagnostic criteria, using SPSS 22.0 and Medcalc data analysis, P <0.05 was considered statistically significant. Rabbit kidneys to take conventional paraffin sections, HE staining observed under light microscope. Results AKI occurred in rabbits in T24, T36 and T48 groups. Compared with those before modeling, the cortical AUCs of PI, T 1/2, mTT, TTP, T36 and T48 in the cortex RT, T24, T36 and T48 of the T12, T24, T36 and T48 groups after modeling And WIS were significantly changed (P <0.05), medullary various imaging parameters did not change significantly. Some angiography indicators of AKI diagnosis better performance, higher sensitivity. Conclusion Contrast-enhanced ultrasound can reflect the changes of early renal cortical perfusion in AKI. The cortical RT can detect AKI earlier than SCr, and has good diagnostic efficacy.