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目的:探讨L-肉碱(LC)在奥硝唑(ORN)所致雄性大鼠生殖系统氧化损伤中的保护作用。方法:40只雄性SD大鼠随机均分为5组,分别连续灌胃20d。A组(对照组):0.5%羧甲基纤维素钠(溶剂);B组:400mg/kgORN;C组:800mg/kgORN;D组:ORN(400mg/kg)+LC(100mg/kg);E组:ORN(800mg/kg)+LC(100mg/kg)。末次给药24h后,麻醉处死所有大鼠,分别检测各组睾丸、附睾组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)水平及总抗氧化能力(TAC)。结果:B组、C组及E组大鼠附睾MDA水平明显高于A组(P均<0.01),睾丸中MDA水平则各组间没有差异;B组、C组及E组睾丸、附睾SOD水平和TAC则分别显著低于A组(P均<0.05),而D组与A组相比无显著性差异;与C组相比,E组大鼠附睾MDA水平显著降低(P<0.01),而TAC则显著升高(P<0.05);虽然E组大鼠附睾、睾丸SOD水平及睾丸中的TAC与C组比无统计学差异,但有升高的趋势。结论:LC可保护大鼠生殖系统对抗ORN所致的氧化损伤,对大鼠生殖功能具有一定的保护作用。
Objective: To investigate the protective effect of L-carnitine (LC) on the oxidative damage of reproductive system in male rats induced by ornidazole (ORN). Methods: Forty male Sprague-Dawley rats were randomly divided into five groups, and were given gavage for 20 days. Group A (control group): 0.5% sodium carboxymethyl cellulose (solvent); Group B: 400mg / kg ORN; Group C: 800mg / kg ORN; Group D: ORN (400mg / kg) Group E: ORN (800 mg / kg) + LC (100 mg / kg). Twenty-four hours after the last administration, all the rats were killed by anesthesia. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and total antioxidant capacity (TAC) were measured in the testis and epididymis of each group. Results: The levels of MDA in epididymis of rats in group B, group C and group E were significantly higher than those in group A (P <0.01), but there was no difference in the level of MDA in testes between groups. In group B, group C and group E, (P <0.05), while there was no significant difference between group D and group A. Compared with group C, the level of epididymal MDA in group E was significantly lower (P <0.01) , While TAC increased significantly (P <0.05). Although the SOD level in epididymis and testes of rats in E group was not significantly different from that of TAC and C groups in testis, there was an increasing trend. Conclusion: LC can protect rat reproductive system against oxidative damage induced by ORN, and has a protective effect on reproductive function in rats.