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本文报告了精液中生精细胞四种鉴别方法。根据生精细胞与体细胞的分裂规律,采用染色体荧光原位杂交技术,根据细胞表面标志,采用鼠抗人CD45、CD3、CD4、CDSMcAb进行识别;根据细胞形态特征,用瑞一姬染色法识别。各实验室可根据条件,选择两种以上检测方法,以提高精液中白细胞检测水平。
This article reports four methods for identifying spermatogenic cells in semen. According to the split rules of spermatogenic cells and somatic cells, chromosomal fluorescence in situ hybridization was used to identify the cell surface markers based on mouse anti-human CD45, CD3, CD4, CDSMcAb. According to the morphological characteristics of the cells, . According to the laboratory conditions, select more than two detection methods to improve the level of white blood cells in semen.