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目的比较新的Cp G岛甲基化表型(CIMP)筛选标记基因和经典CIMP筛选标记基因在CIMP肺癌筛选中的作用,并分析CIMP肺癌的临床病理特征。方法取第二军医大学长海医院呼吸科50例肺癌患者的肺癌组织和癌旁组织,提取DNA,进行甲基化转换后,利用甲基化特异性PCR(MSP)对新的CIMP筛选标记基因(SHISA3、CTSL1、C1ORF103和TMEM200B)和经典的CIMP筛选标记基因(CACNA1G、IGF2、NEUROG1、RUNX3)的启动子Cp G岛区域进行扩增,采用琼脂糖凝胶电泳分析其甲基化状态。运用SPSS统计软件对结果进行统计分析。结果肺癌组织发生明显的甲基化,所研究的8个基因甲基化水平均明显高于癌旁组织(P=0.014)。其中RUNX3甲基化与淋巴结转移及功能状态(PS)评分有关(P值分别为0.017、0.018)。年龄>60岁的肺癌患者甲基化率高于≤60岁者(P=0.031)。吸烟对CTSL1基因甲基化的影响也很大(P=0.018)。结论 Cp G岛甲基化表型肺癌具有独特的临床病理特征;新的和经典的甲基化基因组合在CIMP肺癌筛选上都具有较高的灵敏度和特异性。
Objective To compare the role of novel CpG island methylation phenotype (CIMP) screening marker genes and classical CIMP screening marker genes in CIMP lung cancer screening and to analyze the clinicopathological features of CIMP lung cancer. Methods Lung cancer tissues and adjacent tissues from 50 lung cancer patients in Department of Respiratory, Changhai Hospital, Second Military Medical University were collected and DNA was extracted. After methylation transformation, methylation-specific PCR (MSP) was used to screen the new CIMP screening marker gene SHISA3, CTSL1, C1ORF103 and TMEM200B) and the classical CIMP screening marker genes (CACNA1G, IGF2, NEUROG1, RUNX3) were amplified by PCR, and their methylation status was analyzed by agarose gel electrophoresis. Using SPSS statistical software for statistical analysis of the results. Results Significant methylation of lung cancer tissues was observed. The methylation levels of the 8 genes studied were significantly higher than those in adjacent tissues (P = 0.014). Among them, RUNX3 methylation was associated with lymph node metastasis and functional status (P = 0.017, 0.018, respectively). Patients with lung cancer aged> 60 years had a higher rate of methylation than those ≤60 years of age (P = 0.031). Smoking also had a large effect on the methylation of CTSL1 (P = 0.018). Conclusion CpG island methylation phenotype lung cancer has a unique clinical and pathological features; the combination of new and classic methylation genes in CIMP lung cancer screening has a high sensitivity and specificity.