论文部分内容阅读
目的 了解长期住院老年患者肺部感染铜绿假单胞菌 (PA)的分子流行病学特征 ,为临床防治提供依据。方法 建立 PA随机扩增 DNA多态性 (RAPD)指纹图基因分型方法 ,对与流行病学相关或不相关 34株菌株的基因分型。结果 采用同一反应体系 ,30株临床分离株和 2株不相关株产生稳定 RAPD特异带型 ,分型率为 94 .12 % ,长期住院的同一患者不同时期或不同患者同一时期 PA的 RAPD谱型存在差异。结论 RAPD指纹图基因技术分型率高 ,分辨率强 ,简便快捷 ,可在分子水平上对老年人长期住院感染 PA提供病原学及流行病学的研究方法。
Objective To investigate the molecular epidemiological characteristics of lung-infected Pseudomonas aeruginosa (PA) in elderly patients with long-term hospitalization and provide the basis for clinical prevention and treatment. Methods Genomic DNA was cloned by random amplified polymorphic DNA (RAPD) fingerprinting assay. PA genotypes of 34 isolates that were related to epidemiology or not. Results Using the same reaction system, stable strains of RAPD were detected in 30 clinical isolates and 2 unrelated strains, with a genotyping rate of 94.12%. The long-term hospitalized patients with different types of RAPD profiles at different times or in different periods has a difference. Conclusion The RAPD fingerprinting gene technology has high typing rate, strong resolution, simple and quick method, and can provide etiological and epidemiological research methods for long-term inpatients with PA in the elderly.