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目的:探讨DNA永生化链的分配方式与肿瘤干细胞之间的关系.方法:采用启动和促进两阶段化学诱癌方法在小鼠背部诱导皮肤肿瘤;用溴脱氧尿嘧啶核苷(BrdU)掺入法检测DNA永生化链在发育期、成年期小鼠皮肤成体干细胞及皮肤肿瘤干细胞中的滞留时间;图像分析DNA含量方法检测肿瘤细胞倍体变化.结果:诱癌4mo后,40只小鼠中有17只背部分别长出1～3个皮肤肿瘤.BrdU阳性细胞变化情况检测显示,24h后发育期、成年期标记的小鼠皮肤上皮细胞和肿瘤组织的细胞均为阳性,阳性部位为细胞核;60d后发育期标记的小鼠皮肤上皮中仍有少数细胞为阳性,它们主要存在于毛囊的隆突部位(bulge),少数位于皮肤基底层.标记后30d检测成年期标记的小鼠皮肤上皮细胞,结果均为阴性.标记后30d检测肿瘤组织发现仍有少数细胞BrdU阳性,这些肿瘤细胞主要分布在肿瘤实质组织和间质组织的交界部位;标记后60d检测,肿瘤组织中不再有BrdU阳性细胞.图像分析DNA含量方法发现上述17例小鼠肿瘤都是非整倍体核型.结论:小鼠皮肤成体干细胞存在DNA永生化链,其在有丝分裂过程中发生非随机分配,肿瘤干细胞中可能存在DNA永生化链,其分配方式趋向随机分配.
OBJECTIVE: To investigate the relationship between the distribution of DNA immortalized strands and tumor stem cells.METHODS: Skin tumors were induced on the back of mice using a two-stage chemoattractant method, and bromodeoxyuridine (BrdU) Method was used to detect the residence time of immortalized DNA in developmental and adulthood mouse adult skin stem cells and skin tumor stem cells.Analysis of DNA content by image analysis was used to detect the change of ploidy.Results: There are 17 back of the growth of 1 to 3 skin tumors were detected.BrdU positive cells changes in the test showed that after 24 hours of development, adult mouse skin epidermis and tumor tissue labeled cells were positive, the positive part of the nucleus; After 60 days, a few cells were still positive in mouse skin epithelial cells, which mainly existed in the bulge of hair follicles and a few in the basal layer of the skin. The adult mouse skin epithelial cells , The results were negative.After 30 days of labeling, there was still a few BrdU positive cells in the tumor tissue, which were mainly located in the junction of parenchymal and interstitial tissues 60 days after labeling, BrdU-positive cells were no longer detected in the tumor tissues.Analysis of DNA content showed that all of the 17 mice showed aneuploid karyotype.Conclusion: DNA immortalized DNA strand exists in mouse skin adult stem cells, In the process of mitosis occurs non-random distribution, cancer stem cells may exist DNA immortalized chain, its distribution tends to random distribution.