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以乳腺癌细胞株MCF-7为实验对象,采用细胞增殖抑制率、细胞粘附抑制率、细胞克隆数和细胞迁移距离为评价指标,研究8种食药用菌醇提物对乳腺癌的影响。刺芹侧耳(Pleurotus eryngii)、姬松茸(Agaricus blazei)和灰树花(Grifola frondosa)的醇提物(200μg/mL)处理能显著降低MCF-7细胞的克隆形成数和细胞迁移距离,较阴性对照分别降低了64.3%、64.5%、61.6%和54.8%、54.8%、48.0%,3种醇提物对MCF-7细胞粘附数和细胞增殖的抑制率分别为48.5%、55.1%、49.3%和30.0%、34.1%、29.5%;另外5种食用菌醇提物作用效果不明显。结果初步表明,刺芹侧耳、姬松茸和灰树花的醇提物(200μg/mL)具有一定的体外抑制乳腺癌活性。
Breast cancer cell line MCF-7 was used as experimental object, and the cell proliferation inhibition rate, cell adhesion inhibition rate, cell clone number and cell migration distance were used as evaluation indexes to study the effects of alcohol extracts of 8 kinds of edible fungi on breast cancer . Pleurotus eryngii, Agaricus blazei and Grifola frondosa ethanol extracts (200μg / mL) significantly reduced the number of MCF-7 cells and the migration distance The inhibitory rates of three alcoholic extracts on the adhesion number and cell proliferation of MCF-7 cells were 48.5%, 55.1% and 49.3%, respectively, reduced by 64.3%, 64.5%, 61.6% and 54.8%, 54.8% and 48.0% % And 30.0%, 34.1% and 29.5%, respectively. The effect of the other five edible fungi alcohol extracts was not obvious. The results showed that the alcoholic extract (200μg / mL) of Agkistrodon acutus, Agaricus blazei and Grifola frondosa had a certain inhibitory effect on breast cancer activity in vitro.