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为了进一步探索震颤麻痹的奥秘,本文应用6—羟多巴胺(6—Hydroxydo(?)amine,6—OH—DA)和海人酸(Kainic Acid,KA)两种神经毒在大鼠身上建立了此症的模型。结果当向一侧黑质内注射6—OH—DA选择性损毁多巴胺神经元时,大鼠头和躯干歪向手术侧,向同侧旋转;而向一侧黑质注射海人酸破坏黑质包括DA神经元在内的各类神经元的核周质时,注射后的短时间内顺序出现向手术对侧和同侧歪和旋转的现象,继而两侧旋转交替进行,大约在注药的24小时后,则持续向注药对侧偏转,不再改变方向。在上述动物模型的基础上,皮下注射DA受体激动剂去水吗啡或阻断剂氟哌啶醇,均使一侧SN内注射6—OH—DA的大鼠由向注射的同侧偏转改为向手术对侧偏转。而去水吗啡使一侧SN被KA损毁的大鼠由向损伤的对侧偏转改为同侧偏转;氟哌啶醇使一测SN被KA损毁的大鼠向手术对侧偏转的现象加强。结果提示在黑质网状部可能存在一种神经元系统对SN致密部的DA神经元起抑制作用。当用KA将这类神经也损毁后,大鼠出现的姿势和行为的改变与SN纹体DA系统被损毁后的结果相反。中脑黑质(Substantia Nigra,SN)属于锥体外系的一部分,其主要功能是调节躯体运动。黑质致密部的多巴胺神经元(Dopaminergic Neuron,DAergic Neuron)的变性是震颤麻痹的重要病因。本文应用6—羟多巴胺和海人酸两种神经毒在大鼠身上复制震颤麻痹的动物模型,为进一步探索其病因和治疗奠定基础。
In order to further explore the mystery of Parkinson’s paralysis, this study established this rat neurotoxicity using 6-Hydroxydoamine (6-OH-DA) and Kainic Acid (KA) Symptomatic model. Results When dopamine neurons were selectively damaged by injecting 6-OH-DA into the substantia nigra, the head and trunk of the rats were distorted to the side of the operation and rotated to the same side. When the kainic acid was injected into one side of the substantia nigra and the substantia nigra was damaged Including DA neurons, including the various types of neurons in the perinuclear, shortly after the order of injection to the contralateral and ipsilateral surgical crooked and rotating phenomenon, then turn alternately on both sides, about injection After 24 hours, the drug is continuously deflected towards the opposite side of the drug injection, no longer changing direction. On the basis of the above animal model, subcutaneous injection of the DA receptor agonist dehydromorphine or the blocker haloperidol, both side of the SN injection of 6-OH-DA in rats by injection to the ipsilateral deflection To the operation of the contralateral deflection. While desiccated morphine changed the rats whose one side SN was damaged by KA from the opposite side to the lesion to ipsilateral deflection. Haloperidol enhanced the contralateral deflection of a rat whose SN was damaged by KA. The results suggest that there may be a neuronal system in the substantia nigra reticulum, which inhibits DA neurons in the SN densities. When these nerves were also destroyed by KA, the changes in posture and behavior in rats were reversed to the results after the SN synapse DA system was destroyed. Substantia Nigra (SN) belongs to the extrapyramidal system and its main function is to regulate body movement. Degeneration of dopaminergic neuron (Dopaminergic Neuron) is a major cause of paralysis. In this paper, 6-hydroxydopamine and kainic acid two kinds of neurotoxicity in rats with Parkinson’s disease paraplegia animal model, to further explore the etiology and treatment basis.