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目的:探讨NF-κB与全身炎症反应综合征(SIRS)的关系以及参附注射液的干预作用。方法:本实验设计为3组:正常对照组、脂多糖α(LPS)致伤组及参附注射液(SF)治疗组。采用静脉注射脂多糖(LPS)建立大鼠SIRS模型,并通过检测内毒素筛选成功模型。经相应处理后,各组分别检测不同时间点血液中单个核细胞核因子-κB(NF-κB)、血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平以及肺脏、肝脏组织的病理变化。结果:①LPS致伤后大鼠单个核细胞中NF-κB及TNF-α活性明显增高,2 h最明显(P<0.05),其后逐渐下降;②血清IL-6浓度随着时间的推移不断升高(P<0.05);③病理结果显示,LPS组肺组织出现肺泡出血、水肿,肺泡间隔弥漫性增厚、水肿和炎症细胞浸润;肝脏组织显示肝窦扩张、充血、局灶性肝细胞坏死。SF组肺脏和肝脏的上述损伤出现均明显减轻。SF组和LPS致伤组相比,可显著降低NF-κB活性、TNF-α及IL-6水平(P<0.05),减轻肺脏和肝脏的病理损伤。结论:NF-κB等参与了SIRS的发生、发展,导致肺脏、肝脏损伤;参附注射液可能通过减少NF-κB的激活,减少TNF-α和IL-6的释放,减轻肺脏和肝脏的病理损伤,对SIRS起保护作用。
Objective: To investigate the relationship between NF-κB and systemic inflammatory response syndrome (SIRS) and the intervention of Shenfu injection. Methods: The experiment was designed as 3 groups: normal control group, lipopolysaccharide α (LPS) injury group and Shenfu injection (SF) treatment group. Rat model of SIRS was established by intravenous injection of lipopolysaccharide (LPS), and the successful model was screened by detecting endotoxin. After corresponding treatment, the levels of NF-κB, TNF-α, IL-6 in blood mononuclear cells, Liver tissue pathological changes. Results: ① The NF-κB and TNF-α activity in rat mononuclear cells were significantly increased after LPS injury (P <0.05), and then decreased gradually; ② The serum IL-6 concentration kept increasing with time (P <0.05). The pathological results showed that alveolar hemorrhage, edema, diffuse thickening of alveolar septum, edema and inflammatory cell infiltration in the lung tissue of LPS group. The liver tissue showed hepatic sinusoidal dilation, congestion and focal hepatocyte Necrosis. SF group of lung and liver damage were significantly reduced. NF-κB activity, TNF-α and IL-6 levels in SF group were significantly lower than those in LPS group (P <0.05), and the pathological changes of lung and liver were alleviated. Conclusion: NF-κB and so on are involved in the occurrence and development of SIRS, leading to lung and liver damage. Shenfu injection may reduce the release of TNF-α and IL-6 and reduce the pathological changes of lung and liver possibly through decreasing NF-κB activation Damage, SIRS play a protective role.