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目的了解安徽省多重耐药不动杆菌碳青霉烯类耐药的机制。方法收集安徽省多家医院2006~2007年临床分离非重复的不动杆菌共167株,用琼脂稀释法测定最低抑菌浓度(MICs),用聚合酶链反应(PCR)扩增和克隆测序方法确认碳青霉烯酶基因,用PCR扩增整合酶。结果34株对包括碳青霉烯类抗菌素在内的多种抗菌药耐药,34株中19株产OXA-23型碳青霉烯酶基因、23株Ⅰ类整合酶阳性,未检测到OXA-24型酶,IMP、VIM、SIM型金属酶基因和Ⅱ,Ⅲ整合酶基因。发现2株新基因取得登录号FJ194460;FJ194494。23株I整合酶阳性菌株有21株测出I类整合子基因结构。结论安徽省多重耐药不动杆菌的耐药性与其携带OXA-23型酶和I类整合子有关。
Objective To understand the mechanism of carbapenem resistance in Acinetobacter baumannii in Anhui Province. Methods A total of 167 clinical isolates of Acinetobacter isolated from hospitals in Anhui Province from 2006 to 2007 were collected. The minimum inhibitory concentrations (MICs) were determined by agar dilution method and amplified by polymerase chain reaction (PCR) The carbapenemase gene was confirmed and the integrase was amplified by PCR. Results 34 strains were resistant to various antimicrobial agents including carbapenem antibiotics. Of the 34 strains, 19 strains produced OXA-23 carbapenemase gene and 23 strains of type I integrase were positive. OXA was not detected -24 type enzyme, IMP, VIM, SIM type metalloenzyme gene and Ⅱ, Ⅲ integrase gene. Two new genes were found accession number FJ194460; FJ194494.23 strains I integrase positive strains of 21 strains detected type I integron gene structure. Conclusion The drug resistance of multidrug-resistant Acinetobacter in Anhui Province is related to carrying OXA-23 type I enzyme and class I integron.