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近年来质谱离子化技术方面有两项重要成果:基质辅助激光解吸离子化(matrix-assisted laser desorptionionization,MALDI)和电喷雾离子化(electrospray ionization,ESI)。MALDI和ESI的应用使质谱在生物大分子研究方面取得重大突破。本文仅就MALDI的原理、特点、样品准备方法、基质的选择、仪器条件及其在生物大分子应用方面的最新进展进行简要的综述。
In recent years, there are two important achievements in mass spectrometry ionization: matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI). The use of MALDI and ESI has made mass spectrometry a major breakthrough in the study of biological macromolecules. This article briefly reviews the principles, characteristics, sample preparation methods, matrix selection, instrument conditions and the latest developments in the application of biological macromolecules.