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【目的】分析水稻接种稻瘟病菌(Magnaporthe grisea)48 h后亲和互作与非亲和互作反应的基因表达谱,探索水稻对不同稻瘟病菌抗性差异的分子机理。【方法】运用Affymetrix表达谱芯片分析差异表达mRNA;通过分子注释系统平台(MAS 3.0)对差异表达基因进行了基因注释及GO分析;应用实时定量PCR对部分差异表达基因进行验证。【结果】在49 824个转录本中共检测到大约24 000个转录本,Fold change大于2.5的基因共1 028个,其中非亲和互作上调基因460个,下调基因568个。所验证的4个基因的荧光定量PCR结果与芯片结果基本一致。经GO分析差异基因对应蛋白的功能主要涉及信号转导、酶的调节、转录及分子转运等。【结论】水稻与稻瘟病菌非亲和与亲和互作的基因表达谱存在较大差异,基因芯片筛选到的差异表达基因通过GO注释明确了差异基因的分子功能及信号通路,有利于进一步了解植物抗病机制,并可能为稻瘟病防治措施提供新的途径。
【Objective】 The objective of this study was to analyze the gene expression profiles of the affinity and non-affinity interactions of rice blast inoculated with Magnaporthe grisea for 48 h and to explore the molecular mechanism of rice resistance to different Magnaporthe grisea. 【Methods】 Differential expression of mRNA was analyzed by Affymetrix microarray. Gene annotation and GO analysis of differentially expressed genes were carried out by using Molecular Annotation System (MAS 3.0) platform. Some differentially expressed genes were verified by real-time PCR. 【Result】 A total of 24 000 transcripts were detected in 49 824 transcripts. A total of 1 028 genes with Fold change greater than 2.5 were found, of which 460 were non-affinity interactions and 568 genes were down-regulated. The results of the real-time quantitative PCR of the four genes verified were basically the same as those of the chip. Analysis of GO by differential gene corresponding proteins mainly involves signal transduction, enzyme regulation, transcription and molecular transport. 【Conclusion】 The gene expression profiles of non-affinity and affinity interactions between rice and Magnaporthe grisea are quite different. The differentially expressed genes screened by cDNA microarray confirmed the molecular function and signal pathway of the differential genes by GO annotation, Understand the mechanism of plant disease resistance, and may provide a new way for the prevention and control measures of rice blast.