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目的采用国际通用的SAS统计分析平台编制计算机程序,实现杀虫剂毒力的快速筛选。方法以德国小蠊为试虫,对A、B2种杀虫剂进行室内熏蒸毒力测定,对所得数据进行计算机程序演算,以加权概率单位法拟合毒力回归线,计算2种药剂的LC50及其95%置信限(95%CI),并对2种药剂LC50及毒力回归线斜率进行两两分析比较。结果 A、B药剂杀灭德国小蠊的毒力回归线分别为Y(probit)=-3.193+5.005×Log1(0dose)和Y(probit)=-2.498+2.696×Log1(0dose);参数效应Waldχ2检验的P值均<0.01,方程拟合优度检验的P值均>0.05。2种药剂的LC50值分别为4.35g/m3(95%CI:3.17～5.29g/m3)和8.44g/m3(95%CI:6.07～10.66g/m3);毒力线斜率分别为5.005和2.696。A、B药剂LC50两两比较结果:u=3.415,P<0.001;斜率间两两比较结果:t=2.028,P<0.05。提示从药剂的毒力和虫群敏感性而言,A药剂均强于B药剂。结论建立的快速筛选方法及编制的程序计算结果经双重验证,证明准确、可靠,且简便、快捷。
Objective To prepare a computer program using the internationally accepted SAS statistical analysis platform to achieve rapid screening of insecticide virulence. Methods The Blattella germanica was used as the test insect to test indoor fumigation virulence of A and B2 insecticides. The data were calculated by computer program. The toxicity regression lines were fitted by the weighted probability unit method. The LC50 and The 95% confidence interval (95% CI) was compared between the LC50 of the two agents and the slope of the virulence regression line. Results The virulence regression curves of A and B agents for killing Blattella germanica were Y (probit) = - 3.193 + 5.005 × Log1 (0dose) and Y (probit) = -2.498 + 2.696 × Log1 (P <0.05). The P values of the goodness of fit test of the equation were both> 0.05.The LC50 values of the two kinds of medicaments were 4.35g / m3 (95% CI: 3.17-5.29g / m3) and 8.44g / 95% CI: 6.07 ~ 10.66g / m3). The slopes of virulence lines were 5.005 and 2.696, respectively. A, B Pharmacy LC50 pairwise comparison results: u = 3.415, P <0.001; slope between two comparisons: t = 2.028, P <0.05. Tip from the virulence and insecticidal susceptibilities, A agents are stronger than the B agent. Conclusion The established rapid screening method and the compilation of the results of the program are verified by double verification, which is accurate, reliable and simple and quick.