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生长素响应因子(auxin response factors,ARFs)通过调节下游靶基因广泛参与植物生长发育过程,但ARFs如何调控植物叶片衰老的分子机制还不清楚。该文首先利用实时荧光定量PCR(q PCR)技术,分析大豆生长素响应基因Gm ARF16在叶片自然衰老、人工黑暗诱导衰老、外源植物生长素IAA处理条件下的表达模式,结果表明,该基因与叶片衰老调控密切相关,并且属于生长素的原初响应基因。为了进一步验证Gm ARF16基因的功能,采用农杆菌转化方法分别获得基因敲减(Gm ARF16-RNAi)和抗降解表达(m Gm ARF16)的转基因大豆植株。与非转基因对照相比,Gm ARF16-RNAi转基因大豆植株的叶片叶绿素含量和最大光量子效率(Fv/Fm)显著提高,叶片衰老标记基因(Gm CYSP1)的表达受到抑制,而m Gm ARF16转基因大豆植株则呈现出与Gm ARF16-RNAi转基因大豆植株相反的叶片生理表型。结果表明大豆生长素响应因子Gm ARF16正调节叶片的衰老进程。该研究表明,Gm ARF16在植物生长发育进程中发挥着重要作用。
Auxin response factors (auxin response factors, ARFs) are widely involved in plant growth and development by regulating downstream target genes. However, the molecular mechanisms by which ARFs regulate plant leaf senescence are still unclear. In this study, we first analyzed the expression pattern of Gm ARF16, a natural auxin-induced response to artificial darkness, and auxin IAA by using real-time quantitative PCR (qPCR). The results showed that this gene It is closely related to leaf senescence regulation and belongs to the primary response gene of auxin. In order to further verify the function of Gm ARF16 gene, Agrobacterium transformation methods were used to obtain transgenic soybean plants with gene knockdown (Gm ARF16-RNAi) and anti-degradation expression (m Gm ARF16) respectively. The chlorophyll content and maximum photon quantum efficiency (Fv / Fm) of Gm ARF16-RNAi transgenic soybean plants were significantly increased compared with non-transgenic control plants, and the expression of Gm CYSP1 gene was inhibited. However, m Gm ARF16 transgenic soybean plants The leaf physiological phenotype as opposed to Gm ARF16-RNAi transgenic soybean plants is presented. The results showed that soybean auxin response factor Gm ARF16 positively regulated leaf senescence. This study shows that Gm ARF16 plays an important role in the process of plant growth and development.