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目的 :观察维拉帕米 (verapamil,VP)对三磷酸肌醇 (IP3)引起的大鼠肝细胞钙释放激活的钙电流 (ICRAC)的影响。方法 :采用标准全细胞膜片钳技术。结果 :IP3 激活的ICRAC的峰电流为 (- 0 .2 6± 0 .0 8)nA(n =7) ;5 μmol/L维拉帕米对ICRAC的抑制率为 30 .9%± 7.8% (从 - 0 .2 5± 0 .10nA到 - 0 .17± 0 .0 5nA ,n =5 ,P <0 .0 1)。结果 :维拉帕米能有效抑制IP3 引起的ICRAC。
OBJECTIVE: To observe the effect of verapamil (VP) on calcium current (ICRAC) induced by phosphoinositide (IP3) -induced hepatic calcium release in rats. Methods: Standard whole-cell patch clamp technique was used. Results: The ICRAC peak current of IP3 was (--0.26 ± 0.08) nA (n = 7). The ICRAC inhibition rate of 5 μmol / L verapamil was 30.9% ± 7.8% (From -0.22 ± 0.10 nA to -0.17 ± 0.50 nA, n = 5, P <0.01). Results: Verapamil effectively inhibited IP3-induced ICRAC.