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目的:研究大鼠尾部悬吊模拟失重所致的抑郁样行为学表现并进行初步的机制探讨。方法:利用大鼠模拟失重尾吊14 d,以空场实验、新奇事物实验、新奇抑制摄食实验、强迫游泳和穿梭实验研究大鼠行为学表现,采用酶联免疫分析大鼠血清皮质酮和促肾上腺皮质激素的含量;采用蛋白质印迹法检测大鼠大脑皮层脑源性神经营养因子(BDNF)蛋白的变化。结果:空场试验结果显示,模型组与对照组相比,自主活动能力下降(P<0.05或P<0.01)、爬壁次数减少[模型组(0.70±0.95)次,正常组(10.16±3.78)次,P<0.01],新奇事物和新奇抑制摄食实验潜伏期延长,强迫游泳的不动时间增加,穿梭实验主动穿梭次数减少(P<0.05或P<0.01),血清皮质酮、促肾上腺皮质激素水平升高(P<0.05或P<0.01),大脑皮层中BDNF表达下降(P<0.01)。结论:大鼠尾部悬吊14 d模拟失重可出现抑郁样行为表现。
OBJECTIVE: To study the depressive-like behaviors induced by simulated weightlessness in the tail suspension of rats and the preliminary mechanism study. Methods: Rats were used to simulate the weight-loss tail suspension for 14 days. The behavior of the rats was studied by air field experiment, novelty experiment, novel inhibition feeding experiment, forced swimming and shuttle experiment, and the serum corticosterone was analyzed by enzyme-linked immunosorbent assay Cortisol levels in the cerebral cortex were detected by Western blotting. The changes of brain-derived neurotrophic factor (BDNF) protein in rats were detected by Western blot. Results: The results of field test showed that compared with the control group, the autonomic ability decreased (P <0.05 or P <0.01) and the number of climbing walls decreased in model group (0.70 ± 0.95) and in normal group (10.16 ± 3.78 ), P <0.01]. The latency of novelty and novelty in feeding experiment was prolonged, the immobility time of forced swimming increased, the number of active shuttle in shuttle experiment decreased (P <0.05 or P <0.01), serum corticosterone, adrenocorticotrophic hormone (P <0.05 or P <0.01). The expression of BDNF in cerebral cortex decreased (P <0.01). CONCLUSION: Depression-like behavior may appear on simulated weightlessness after 14-day tail suspension in rats.