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本实验以犬急性轻型和重型胰腺炎模型为对象，研究重症胰腺炎早期血清学变化。健康杂种犬１８只随机分三组。Ⅰ组为对照组，Ⅱ，Ⅲ组分别用１．５％，５％牛磺胆酸钠溶液加压注入犬的主胰管制成犬的轻型和重型胰腺炎模型，手术后１ｈ，４ｈ，７ｈ，１２ｈ取静脉血检测淀粉酶，脂肪酶，磷脂酶Ａ２（ＰＬＡ２）和正铁血红白蛋白（ＭＨＡ）的含量。结果发现重症组犬血ＰＬＡ２在术后４ｈ活性明显升高，为轻型组的１．８倍（Ｐ＜０．０１），对照组的４．７倍（Ｐ＜０．０１），且随病程的延长活性逐渐升高。ＭＨＡ的检测采用双光束二导波紫外分光光度法，血清不需任何处理，敏感性高，１μｍｏｌ／Ｌ以上的含量即可测出。本实验结果表明：重症组犬血ＭＨＡ含量在术后１ｈ即明显升高１７．１２±４．６１μｍｏｌ／Ｌ，为轻型的４．１倍（Ｐ＜０．０１），对照组的５．３倍（Ｐ＜０．０１），并且发现在术后１２ｈ时ＭＨＡ含量突然增高为轻型组的１１．７倍（Ｐ＜０．０１）对照组的３９．１倍（Ｐ＜０．０１）。重症组淀粉酶活性仅在１ｈ，４ｈ时与轻型组有显著性差异（Ｐ＜０．０５，Ｐ＜０．０１），而后随病程的延长其活性反而下降。脂肪酶活性升高的较早，术后１ｈ即与轻型组有显著性差别（Ｐ＜０．０?
In this study, acute canine and severe acute pancreatitis model canine for the study of early Serious changes of severe pancreatitis. 18 healthy mongrel dogs were randomly divided into three groups. The rats in group Ⅰ and Ⅱ were injected with 1.5% and 5% sodium taurocholate solution respectively into the canine model of mild and severe pancreatitis. After 1h, 4h, 7h Venous blood samples were taken for detection of amylase, lipase, phospholipase A2 (PLA2) and methemoglobin (MHA) at 12 h. The results showed that the activity of PLA2 in critically ill dogs at 4 hours after operation was significantly higher than that in the mild group (P <0.01), 4.7 times that in the control group (P <0.01) Increased activity of the gradual increase. MHA detection using dual-beam two-guided UV spectrophotometry, serum without any treatment, high sensitivity, 1μmol / L or more content can be measured. The results of this experiment showed that the levels of MHA in critically ill dogs were significantly increased by 17.12 ± 4.61μmol / L at 1 hour after surgery, 4.1 times more than those with mild (P <0.01) and 5.3 (P <0.01), and found that the content of MHA increased suddenly at 12h after operation to 11.7 folds (P <0.01) in the light group and 39.1 folds (P <0.01) in the control group. The activity of amylase in severe group was significantly lower than that in the light group at 1 hour and 4 hours (P <0.05, P <0.01), but decreased with the prolongation of the course. Lipase activity increased earlier, 1h after operation and light group was significantly different (P <0.0?