生物技术是目前世界上最活跃、最令人鼓舞的前沿高新科技之一,它将在解决全人类所面临的能源危机、粮食紧缺和环境污染等方面发挥主导作用。以植物生物技术为基础的农业分子育种,为培育高产、优质、抗病虫及抗逆境的新品种或新品系提供了一种有效的手段,它有许多为传统的常规育种所不具备的优势。运用植物生物技术在理论上可以将各种来源的目的基因通过一定途径在植物受体细胞内实现遗传转化,从而达到改良作物遗传性状,培育优良品种的目的。这个外源基因在植物细胞中表达的整个技术路线包括:目的基因的分离和克隆;目的基因在受体细胞内实现载体或非载体介导的基因转移; Biotechnology is one of the most active and the most up-to-date hi-techs in the world. It will play a leading role in solving the energy crisis, food shortage and environmental pollution facing all mankind. Plant molecular breeding based on plant biotechnology provides an effective means of cultivating new varieties or lines with high yield, good quality, resistance to pests and diseases, and has many advantages not available for traditional conventional breeding . The use of plant biotechnology in theory can be a variety of sources of the target gene through a certain way in plant receptor cells to achieve genetic transformation, so as to achieve the purpose of improving crop genetic traits, cultivating fine varieties. The entire technical route for the expression of this exogenous gene in plant cells includes the isolation and cloning of the gene of interest, the achievement of vector-mediated or non-vector-mediated gene transfer of the gene of interest in the recipient cell,